| 质粒名称: | pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002173 |
| 产品用途: | Vector containing the sgRNA scaffold–hCtRNA amplification template |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PGK |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA-Scaffold-hCtRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR载体质粒基本信息
pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR质粒图谱载体图谱和pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR载体序列质粒序列多克隆位点信息
pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR质粒载体简介
pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR质粒序列载体序列
LOCUS pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR 4718 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4718)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4718
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 48..198
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 124..198
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(31..47)
/label=OL459
misc_RNA 48..123
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 48..88
/label=OL462
primer_bind complement(106..142)
/label=OL463
misc_feature 124..198
/label=hCtRNA
primer_bind 124..198
/label=OL409
primer_bind complement(124..198)
/label=OL410
primer_bind 139..162
/label=OL464
primer_bind complement(181..202)
/label=OL465
primer_bind 199..218
/label=OL458
misc_feature 237..354
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 403..913
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 935..1534
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 1681..1802
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2221..2809)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2980..3840)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3841..3945)
/gene="bla"
/label=AmpR promoter
rep_origin 3972..4427
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4558..4606
/note="synthetic polyadenylation signal"
misc_feature 4620..4711
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactaggtt ttagagctag
61 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
121 tgcagagggg gtatagctca gtggtagagc atttgactgc agatcaagag gtccccggtt
181 caaatccggg tgcccccttt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
241 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
301 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
361 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
421 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
481 acgcagcggc gccgaccctg ggactcgcac attcttcacg tccgttcgca gcgtcacccg
541 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
601 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
661 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
721 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
781 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
841 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
901 cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg
961 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
1021 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
1081 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
1141 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
1201 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
1261 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
1321 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
1381 ggcggccgag cgcgccgggg tgcccgcctt cctggaaacc tccgcgcccc gcaacctccc
1441 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
1501 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
1561 gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga cttacaaggc
1621 agctgtagat cttagccact ttctagagtc ggggcggccg gccgcttcga gcagacatga
1681 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
1741 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
1801 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
1861 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
1921 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
1981 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
2041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
2101 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
2161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
2221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
2281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
2341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
2401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
2461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
2521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
2581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
2641 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
2701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
2761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
2821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
2881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
2941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
3001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
3061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
3121 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
3181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
3241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
3301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
3361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
3421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
3481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
3541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
3601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
3661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
3721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
3781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
3841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
3901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
3961 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4021 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
4081 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4141 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
4201 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4261 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
4321 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
4381 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt
4441 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
4501 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
4561 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
4621 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
4681 agtgcaagtg caggtgccag aacatttctc tatcgata
//
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pGL3-sgRNA Scaffold-hCtRNA-PGK-puroR质粒载体应用举例
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